| 132 | Magdalena Kristo (UWEC) def. Cosette Gaines (COEW) 6-0, 6-0 |
| 132 | Aisha Gurung (COEW) def. Samantha Behling (CUW) 6-0, 6-4 |
| 132 | Alice Tysoe (COEW) def. Val Garrido (CSS) 4-6, 6-0, 10-5 |
| 64 | Alli Hagness (COEW) def. Brooke Lindeman (UWRF) 6-4, 6-4 |
| 64 | Sophia Fain (COEW) def. Emmie Kinder (CORNELL) 4-6, 6-4, 10-7 |
| 64 | Addison Leasure (COEW) def. Payton Douglas (BETHEL) 6-3, 7-5 |
| 64 | Aisha Gurung (COEW) def. Vanessa Pham (HAMLINE) 6-2, 6-1 |
| 64 | Alice Tysoe (COEW) def. Clara Hills (LAWRENCE) 6-7 (0-2), 7-5, 10-7 |
| 32 | Abigail Weaver (UWW) def. Alli Hagness (COEW) 6-0, 6-0 |
| 32 | Chloe Regal (UWSP) def. Addison Leasure (COEW) 6-2, 4-6, 10-2 |
| 32 | Ella DeYoung (GAC) def. Aisha Gurung (COEW) 6-0, 6-1 |
| 32 | Noor Omar (GAC) def. Sophia Fain (COEW) 4-6, 6-3, 10-7 |
| 32 | Rory Wahlstrand (GAC) def. Alice Tysoe (COEW) 6-2, 6-1 |
| - | Gustie Invite Draw 1 |
| 32 | Lucille Rozeboom (COEW) def. M. Diefenthaler (STNORBER) 4-1, 4-1 |
| 16 | Lucille Rozeboom (COEW) def. Allie Brosteau (UWSP) 4-0, 4-1 |
| 8 | Annie Klemmensen (GAC) def. Lucille Rozeboom (COEW) 5-3, 4-0 |
| - | Gustie Invite Draw 4 |
| 32 | Carlie Miller (COEW) def. Claire Erdal (STBEN) 4-2, 4-1 |
| 16 | Carlie Miller (COEW) def. Irene Multhauf (STNORBER) 4-0, 4-2 |
| 8 | Clara LeConte (LORAS) def. Carlie Miller (COEW) 4-5 (0-4), 4-1, 10-5 |
